The allergenic non-specific lipid transfer protein from peach: structural studies
نویسندگان
چکیده
منابع مشابه
کلون کردن ژن ns-ltp (non-specific lipid-transfer protein) برنج با برچسب gst به داخل باکتری اشرشیا کلی
پروتئین های ناقل لیپید (ltps) گروهی از پروتئین ها با وزن ملکولی متوسط در نزد گیاهان هستند که دارای پتانسیل های کاربردی به ویژه در سیستم های رها سازی دارو می باشند. هدف از این تحقیق، کلون کردن ژن ltp2 مربوط به گیاه برنج(oryza sativa) در وکتور بیانی pgex-6p-2، در باکتری e.coli (top10) جهت تولید پروتئین به صورت متصل با gst می باشد. از آنجائیکه ژن ltp2 در گیاه برنج را فاقد اینترون است این ژن با کمک...
15 صفحه اولNon-specific lipid transfer proteins: a protein family in search of an allergenic pattern.
committee. Sequence identity between the three mature protein sequences is quite low, ranging from 66 to 68%. These isoforms demonstrated 62–65% sequence identity to Pru p 3. Interestingly, higher sequence identities were observed with Gossypium spp. (72%), Sorghum bicolor (67%) and Zea mays (66%). Although the study enrolled only a limited number of pomegranate-allergic patients (n = 6), surpr...
متن کاملReview. Plant food allergens: peach non-specific lipid transfer protein Pru p 3 as a model
Plant non-specific lipid transfer proteins (LTPs) are plant defence proteins that constitute a relevant panallergen family present in both plant foods and pollens. Their high resistance to proteolytic digestion, thus being probably primary sensitizers by ingestion, and association with systemic and severe clinical symptoms, have led to propose LTPs as a model of true food allergens. Peach Pru p...
متن کاملThe non-specific lipid transfer protein from hazelnut, Cor a 8, a relevant food allergen
Methods nCor a 8 was purified from raw hazelnuts by precipitation and chromatographical steps. Subsequently, the protein was identified by N-terminal sequencing and mass spectrometry. Stability of the protein was investigated by simulated gastric and duodenal digestion assays. Hazelnut lipids were extracted employing the hexane method. Finally, IgE binding activity of native and reduced/alkylat...
متن کاملStructural basis of non-specific lipid binding in maize lipid-transfer protein complexes revealed by high-resolution X-ray crystallography.
Non-specific lipid-transfer proteins (nsLTPs) are involved in the movement of phospholipids, glycolipids, fatty acids, and steroids between membranes. Several structures of plant nsLTPs have been determined both by X-ray crystallography and nuclear magnetic resonance. However, the detailed structural basis of the non-specific binding of hydrophobic ligands by nsLTPs is still poorly understood. ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2005
ISSN: 0108-7673
DOI: 10.1107/s0108767305089257